Pro-Mag PLM-2000 — LAMP Extraction Protocol Guide (magnetic bead vs. column)
LAMP requires higher RNA purity than standard PCR — Bst DNA polymerase is more sensitive to inhibitors than Taq. This guide explains why magnetic bead chemistry outperforms columns for LAMP, how Pro-Mag PLM-2000 is validated for that workflow, and what RNase-free QC actually buys you at the bench.
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Why columns underperform for LAMP
| Variable | Silica column | Magnetic bead (PLM-2000) |
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| Inhibitor carryover (heme, humic acid, protein) | Higher — co-purifies during binding | Lower — bead wash discrimination is more aggressive |
| Centrifugation steps | 3–5 spins per sample | None — magnetic separator |
| Throughput on 96-sample run | Centrifuge bottleneck — serial | Parallel — one rack, all samples |
| Bst polymerase compatibility | Validation often PCR-only | Validated for LAMP |
| RNase-free QC on every buffer lot | Varies by vendor | Yes — tested at lot release |
Inhibitors that reduce PCR efficiency (and you can recover from with extra cycles) will completely block LAMP. The validation point is not theoretical — it determines whether your reaction returns a result.
PLM-2000 protocol — the four steps
- Lyse: Add lysis buffer + sample to tube; incubate per IFU. Lysis disrupts cells and inactivates RNases.
- Bind: Add silica-coated magnetic beads + binding buffer. RNA binds the bead surface in chaotropic salt.
- Wash: Place tube on magnetic separator (PLM-2001/2002/2003). Beads pellet against tube wall; aspirate supernatant. Wash with provided buffers — protein and inhibitor carryover is removed at this step. No centrifuge required.
- Elute: Add low-salt elution buffer; remove from magnet; transfer eluate (RNA) to clean tube. Eluate is direct input to your LAMP reaction.
Total RNA isolated within minutes. Lysis-to-elution is faster than column-based methods because there is no centrifugation wait time at any step.
Sample compatibility
Validated across respiratory specimens (nasopharyngeal swabs, nasal swabs, BAL), whole blood, serum, plasma, urine, stool, tissue, and environmental samples. Contact tech support for sample-specific input volumes.
LAMP platform compatibility
- Optigene Genie II / Genie III / Genie HT — PLM-2000 eluate is direct LAMP-reaction input (both platforms available on pro-lab-direct.com)
- Standard real-time PCR cyclers running isothermal detection chemistries
- RT-PCR (when reverse-transcription master mix is added separately)
Magnetic separator racks — required, sold separately
| SKU | Format |
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| PLM-2001 | Single-tube / low-throughput rack |
| PLM-2002 | 8-strip / 24-tube rack |
| PLM-2003 | 96-well plate-format rack |
If your lab already owns a magnetic separator from another application, contact tech support to confirm compatibility before ordering.
Specifications
- SKU: PLM-2000 · 100 preparations per kit · quote-based pricing
- Validated for LAMP, real-time PCR, RT-PCR
- RNase-free QC on every buffer lot
- ISO 13485 manufactured
Reply for the IFU PDF, sample-type-specific input volumes, or a quote for PLM-2000 + matched separator rack.
View this document online: https://www.pro-lab-direct.com/pro-mag/deliverables/lamp-protocol-guide.html
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