RUO: PL.5902-80 · PL.5902-125 · PL.5902-1000 · PCR IVD: PL.5902-80PCR · PL.5902-125PCR · PL.5902-1000PCR

Nuclease-Free Water — DNase/RNase-Free, DEPC-Free, Per-Lot Release Tested

Name: DNase/RNase Free Water
Brand: Pro-Lab Diagnostics
SKU: PL.5902-1000

125 mL, 1 L, and 80 × 2 mL aliquot formats for PCR, RT-PCR, qPCR, and enzyme reaction setup. Available in Research Use Only (RUO) and PCR IVD grades. Every lot is tested for absence of DNase and RNase activity. No DEPC.

RUO from $31.36 · 1 L bottle $95.36 See all formats and prices

First paid order: 25% off the prices shown when you select the offer at checkout. RUO 125 mL becomes $23.52; RUO 1 L becomes $71.52, before shipping and tax. One use per institution; cannot be combined with coupons or contract pricing.

Order Free 125 mL Sample

Need manager or purchasing approval? Add your format to the cart, then send the cart summary for review or complete checkout with a PO number.

DEPC-Free

Proprietary process

Per-Lot QC

DNase + RNase release

3 Pack Formats

125 mL, 1 L, or 80 × 2 mL

RUO + PCR IVD

Choose the grade for your workflow

Try it in your workflow

Order a free 125 mL RUO sample

Request one no-charge DNase/RNase-Free Water evaluation sample for your US laboratory. No payment is collected. Our team will confirm availability and shipping after your order is placed.

One sample per institution in 90 days. Research use only. The sample is the 125 mL RUO catalog format (PL.5902-125), provided at no charge after staff confirm availability.

Lock in supply and pricing

Request a 6, 12, or 24 month price lock in the volume quote form. A 12 or 24 month standing order can lock in a 25% discount from the listed product price after we agree on volumes and delivery cadence.

Quote requests are subject to a written agreement stating the selected SKUs, unit prices, minimum volume, delivery schedule, effective date, and signatures. First order and standing order offers do not stack.

Why DEPC-Free Matters

DEPC (diethylpyrocarbonate) is the most common chemical treatment for nuclease removal — but DEPC residue is a documented inhibitor of downstream enzyme reactions, especially reverse transcription and in vitro translation. Most autoclave protocols are designed to convert DEPC to ethanol and CO₂, but trace residue is a recurring debate in molecular biology literature.

Pro-Lab's process removes nucleases without DEPC — designed for labs running sensitive RT-PCR, qPCR, IVT, or library-prep workflows where residual chemistry could matter.

Per-Lot Release Testing

Every lot is manufactured under controlled conditions and tested to confirm absence of detectable DNase and RNase activity. Testing uses validated enzymatic assays with sensitivity appropriate for trace nuclease detection. Only lots that meet internal release specifications are approved for distribution.

✓Per-lot DNase + RNase release testing — validated enzymatic assays
✓Lot # and expiry on every container — full traceability for CAP / CLIA / ISO 13485 documentation
✓Lot identified — lot number and expiry are printed on each container

Specs at a Glance

Catalog #

RUO: PL.5902-80, PL.5902-125, PL.5902-1000 · PCR IVD: PL.5902-80PCR, PL.5902-125PCR, PL.5902-1000PCR

Pack formats

125 mL bottle · 1 L bottle · 80 × 2 mL aliquot box

Storage

15–30 °C (room temperature)

DNase activity

Not detectable (per-lot release tested)

RNase activity

Not detectable (per-lot release tested)

DEPC treatment

None — DEPC-free process

Lot # + expiry

Printed on every container

CAS No.

7732-18-5

OSHA HCS classification

Not classified per 29 CFR 1910.1200

Hazard pictogram

None — not applicable

Grades

Research Use Only (RUO) and PCR IVD Grade — per-lot release tested

How It Compares

Spec	Pro-Lab PL.5902 series	Thermo Ambion (AM99xx)	Invitrogen UltraPure (10977)	IDT (11-05-01-14)	In-House Milli-Q + DEPC
DEPC-free	Yes	No (DEPC-treated)	Yes	Yes	Usually DEPC-treated
Per-lot release testing	Yes (IFU)	Production-level	Per lot	Per lot	None
Pack format	1 L	50 mL → 100 mL	500 mL · 6×500 mL	50 mL · 1 L	DIY
Lot # + expiry on container	Yes	Yes	Yes	Yes	No
Factory direct	Yes — Texas	Distributor	Distributor	Yes — Iowa	n/a
CoA per lot	On request	Cert Tracker	On request	On request	None

Competitor data snapshot 2026-05; re-verified quarterly. Public catalogs + datasheets.

Pricing

RUO Grade

36% off · Research Use Only

125 mL bottle

PL.5902-125

$49.00

$31.36

1 L bottle

PL.5902-1000

$149.00

$95.36

80 × 2 mL aliquot box

PL.5902-80

$199.00

$159.20

PCR IVD Grade

PCR IVD

125 mL bottle

PL.5902-125PCR

$59

1 L bottle

PL.5902-1000PCR

$179

80 × 2 mL aliquot box

PL.5902-80PCR

$249

Prices shown in USD. Shipping and applicable tax are calculated at checkout.

Buying through purchasing?

Add the needed format to your cart, then send its summary to purchasing for approval. PO checkout is also available.

Compliance & Documentation

IFU — DNase/RNase Free Water product information sheet, revision 2026-05-1. Included with shipment.
SDS — DNase/RNase Free Water Safety Data Sheet, v1.0 (5/5/2026), conforms to OSHA HCS 29 CFR 1910.1200 (revised 2024).
Hazard status (US): Not classified per HCS — no pictogram, no signal word, no hazard or precautionary statements required.
Manufacturer: Pro-Lab Diagnostics, with ISO 13485 manufacturing.
Grade: Manufacturing-grade molecular biology water — built for production workflows, library prep, NGS, and QC labs.

FAQ

Is this water DEPC-treated?

No. Pro-Lab's process removes nucleases without DEPC or other chemical treatments.

Is every lot tested?

Yes — each lot is tested for absence of detectable DNase and RNase activity using validated enzymatic assays before release.

How should I store it?

15–30 °C (room temperature), in the original container, tightly closed and protected from contamination.

Is this suitable for production / manufacturing / NGS workflows?

Yes. This is manufacturing-grade molecular biology water — designed for production-scale PCR / RT-PCR / qPCR, NGS library prep, QC labs, and in-line reagent prep. Every lot is release-tested for DNase and RNase activity; lot number and expiry are printed on every container; CoA is available on request by lot.

What lot documentation is supplied?

The IFU confirms lot-release testing for DNase and RNase activity, and the lot number and expiry are printed on the container. Contact support@pro-lab.us to ask which additional controlled documents are available for a specific lot.

Do you offer volume pricing?

Yes. Request 6, 12, or 24 month price lock terms with your expected volume and delivery cadence in the quote form. A 12 or 24 month standing order can qualify for 25% off listed prices after written approval.

How do I get the free sample or first order discount?

Use the free 125 mL RUO sample form on this page. For a first paid order, select the 25% offer in checkout. The offer is limited to one use per institution and cannot be combined with coupons or contract pricing.

Do you ship internationally?

Contact Pro-Lab Direct for current US availability. Canadian customers should contact the Canadian office for RUO and PCR IVD grade pricing, volume pricing, availability, and ordering terms. For the UK, contact the applicable Pro-Lab regional office before ordering.